Human schistosomiasis is a neglected tropical disease that results from infection by several species of the parasitic flatworm Schistosoma. My research is focused on defining and characterizing the molecular basis for innate resistance and susceptibility of inbred strains of the snail Biomphalaria glabrata to infection by larvae of the human blood fluke Schistosoma mansoni, causative agent of hepatosplenic schistosomiasis. Methods involving cellular and molecular biology, tissue culture, genomics/proteomics and whole organism manipulations are used to define and characterize the innate immune system of the snail host, in order to gain a better understanding of the underlying processes regulating immune resistance to schistosome larval infection.
Tim Yoshino, PhD
2115 Observatory Dr
Madison, WI 53706
PhD, Biology, University of California, Santa Barbara
Postdoctoral, Institute for Pathobiology, Lehigh University
Molecular Parasitology, Schistosomiasis, Parasite-Host Interactions, Mollusc Intermediate Host, Innate Immunity, Pathogen Recognition Receptors, Glycobiology, Neglected Tropical Diseases
Yoshino, T.P., Wu, X.J., Liu, H.D., Gonzalez, L.A., Deelder, A.M., Hokke, C.H. 2012. Glycotope sharing between snail hemolymph and larval schistosomes: Larval transformation products alter shared glycan patterns of plasma proteins. PLoS Negl Trop Dis (6(3):e1569.
Yoshino, T. P. and C. Coustau. 2011. Immunobiology of Biomphalaria-Trematode Interactions. In: “Biomphalaria Snails and Larval Trematodes” (R. Toledo, B. Fried, eds), Chapt. 7, p. 159-189. Springer, New York
Taft, A.S. and T.P. Yoshino. 2011. Cloning and functional characaterization of two calmodulin genes during larval development in the parasitic flatworm Schistosoma mansoni. J. Parasitol. 97:72-81.
Taft, A.S., Norante, F.A. and T.P. Yoshino (2010) The identification of inhibitors of Schistosoma mansoni miracidial transformation by incorporating a medium-throughput small-molecule screen. Exp Parasitol. 125:84-95.
Yoshino, T.P., N. Dinguirard, and M.M. Mourão. (2010). In vitro manipulation of gene expression in larval Schistosoma: a model for postgenomic approaches in Trematoda. Parasitology 137:463-483.
Fitzpatrick, J.M., E. Peak, S. Perally, I.W. Chalmers, J. Barrett, T.P. Yoshino, A.C. Ivens and K.F. Hoffmann. (2009). Anti-schistosomal candidate molecules identified by lifecycle transcriptomic analyses. PLoS Negl. Trop. Dis. 3(11):e543.
Peterson, N. A., Hokke, C.H., Deelder, A.M. and Yoshino, T.P. (2009). Glycotope analysis in miracidia and primary sporocysts of Schistosoma mansoni: differential expression during the miracidium-to-sporocyst transformation. Intern. J. .Parasitol. 39:1331-1344.
Mourão M.M., Dinguirard N., Franco G.R. and Yoshino T.P. (2009) Role of the endogenous antioxidant system in the protection of Schistosoma mansoni primary sporocysts against exogenous oxidative stress. PLoS Negl. Trop. Dis. 3(11):e550.
Mourão, M.M., N. Dinguirard , G.R. Franco and T.P. Yoshino (2009). Phenotypic screen of early- developing larval blood flukes, Schistosoma mansoni, using RNA interference. PLoS Negl. Trop. Dis. 3(8):e502.
Taft, S.A., J.J. Vermeire, J. Bernier, S.R. Birkeland, M.J. Cipriano, A.R. Papa, A.G. McArthur, and T.P. Yoshino (2009). Transcriptome analysis of Schistosoma mansoni larval development using serial analysis of gene expression (SAGE). Parasitology 136:1-18.