Nader Sheibani, PhD

Ophthalmology and Visual Sciences
1111 Highland Avenue
9453 Madison WI 53705
(608) 263-3345
Focus Groups: 
Signal Transduction
PhD, University of Nebraska Medical Center
Research Summary: 
The role of cell-cell and cell-matrix interactions in vascular development and neovascularization
Research Detail: 

Our laboratory studies the molecular and cellular mechanisms that regulate vascular development and angiogenesis. We are interested in how alterations in normal regulatory mechanisms, under pathological conditions such as ischemia, diabetes, and aging, lead to vascular dysfunction and growth of new blood vessels. We use the postnatal developing retinal vasculature as a model to study all aspects of vascular development. In addition, we have established a number of mouse models to study neovascularization associated with aging, ischemia and diabetes. Our in vivo studies are complemented with our unique ability to culture various retinal vascular cells from wild type and transgenic mice for in vitro mechanistic studies. The utilization of these models has provided novel insight into the role of a number of genes in regulation of vascular development and neovascularization. We are also utilizing our vascular cells for development of cell-based assays for identification of small molecules with potential therapeutic benefit for various diseases with a neovascular component. We are also working with our colleagues in the department of Biomedical Engineering on developing vascular mimetic three dimensional units using defined synthetic materials for high throughput screening of chemicals with adverse effects on vascular function, as well as identifying potential novel pro- and/or anti-angiogenic agents 

Selected Publications: 
Mechanistic role of cytochrome P450 (CYP)1B1 in oxygen-mediated toxicity in pulmonary cells: A novel target for prevention of hyperoxic lung injury. Dinu D, Chu C, Veith A, Lingappan K, Couroucli X, Jefcoate CR, Sheibani N, Moorthy B. Biochem Biophys Res Commun. 2016 May 25. pii: S0006-291X(16)30840-3. doi: 10.1016/j.bbrc.2016.05.125. PMID: 27235555
Quantitative Assessment of Retinopathy Using Multi-parameter Image Analysis. Ghanian Z, Staniszewski K, Jamali N, Sepehr R, Wang S, Sorenson CM, Sheibani N, Ranji M. J Med Signals Sens. 2016 Apr-Jun;6(2):71-80. PMID: 27186534
Differential regulation of angiogenesis using degradable VEGF-binding microspheres. Belair DG, Miller MJ, Wang S, Darjatmoko SR, Binder BY, Sheibani N, Murphy WL. Biomaterials. 2016 Jul; 93:27-37. doi: 10.1016/j.biomaterials.2016.03.021. Epub 2016 Mar 16. PMID: 27061268
Ocular Safety of Intravitreal Propranolol and Its Efficacy in Attenuation of Choroidal Neovascularization. Nourinia R, Rezaei Kanavi M, Kaharkaboudi A, Taghavi SI, Aldavood SJ, Darjatmoko SR, Wang S, Gurel Z, Lavine JA, Safi S, Ahmadieh H, Daftarian N, Sheibani N. Invest Ophthalmol Vis Sci. 2015 Dec;56(13):8228-35. doi: 10.1167/iovs.15-17169. PMID: 26720475
Visible light optical coherence tomography measures retinal oxygen metabolic response to systemic oxygenation. Yi J, Liu W, Chen S, Backman V, Sheibani N, Sorenson CM, Fawzi AA, Linsenmeier RA, Zhang HF. Light Sci Appl. 2015 Sep;4(9). pii: e334. Epub 2015 Sep 25. PMID: 26658555
Functional role of inorganic trace elements in angiogenesis part III: (Ti, Li, Ce, As, Hg, Va, Nb and Pb). Saghiri MA, Orangi J, Asatourian A, Sorenson CM, Sheibani N. Crit Rev Oncol Hematol. 2016 Feb;98:290-301. doi: 10.1016/j.critrevonc.2015.10.004. Epub 2015 Oct 20. Review. PMID: 26638864
Lipopolysaccharide-induced blood-brain barrier disruption: roles of cyclooxygenase, oxidative stress, neuroinflammation, and elements of the neurovascular unit. Banks WA, Gray AM, Erickson MA, Salameh TS, Damodarasamy M, Sheibani N, Meabon JS, Wing EE, Morofuji Y, Cook DG, Reed MJ. J Neuroinflammation. 2015 Nov 25;12:223. doi: 10.1186/s12974-015-0434-1. PMID: 26608623
Cone-Specific Promoters for Gene Therapy of Achromatopsia and Other Retinal Diseases. Ye GJ, Budzynski E, Sonnentag P, Nork TM, Sheibani N, Gurel Z, Boye SL, Peterson JJ, Boye SE, Hauswirth WW, Chulay JD. Hum Gene Ther. 2016 Jan;27(1):72-82. doi: 10.1089/hum.2015.130. PMID: 26603570
Endothelium Expression of Bcl-2 Is Essential for Normal and Pathological Ocular Vascularization. Zaitoun IS, Johnson RP, Jamali N, Almomani R, Wang S, Sheibani N, Sorenson CM. PLoS One. 2015 Oct 7;10(10):e0139994. doi: 10.1371/journal.pone.0139994. eCollection 2015. PMID: 26444547
Topiramate Protects Pericytes from Glucotoxicity: Role for Mitochondrial CA VA in Cerebromicrovascular Disease in Diabetes. Patrick P, Price TO, Diogo AL, Sheibani N, Banks WA, Shah GN.J Endocrinol Diabetes. 2015;2(2). pii: PMID: 26167540
Functional role of inorganic trace elements in angiogenesis-Part II: Cr, Si, Zn, Cu, and S. Saghiri MA, Asatourian A, Orangi J, Sorenson CM, Sheibani N. Crit Rev Oncol Hematol. 2015 Oct;96(1):143-55. doi: 10.1016/j.critrevonc.2015.05.011. Epub 2015 May 27. Review. PMID: 26088455
Functional role of inorganic trace elements in angiogenesis--Part I: N, Fe, Se, P, Au, and Ca. Saghiri MA, Asatourian A, Orangi J, Sorenson CM, Sheibani N. Crit Rev Oncol Hematol. 2015 Oct;96(1):129-42. doi: 10.1016/j.critrevonc.2015.05.010. Epub 2015 Jun 16. Review. PMID: 26088454
Cytochrome P450 1B1 and Primary Congenital Glaucoma. Zhao Y, Sorenson CM, Sheibani N. J Ophthalmic Vis Res. 2015 Jan-Mar;10(1):60-7. doi: 10.4103/2008-322X.156116. Review. PMID: 26005555
PECAM-1 isoforms, eNOS and endoglin axis in regulation of angiogenesis. Park S, Sorenson CM, Sheibani N. Clin Sci (Lond). 2015 Aug;129(3):217-34. doi: 10.1042/CS20140714. Review. PMID: 25976664
Thrombospondin-2 Expression During Retinal Vascular Development and Neovascularization. Fei P, Palenski TL, Wang S, Gurel Z, Hankenson KD, Sorenson CM, Sheibani N. J Ocul Pharmacol Ther. 2015 Sep;31(7):429-44. doi: 10.1089/jop.2014.0151. Epub 2015 May 7.PMID: 25950258
Diabetes and retinal vascular dysfunction. Shin ES, Sorenson CM, Sheibani N. J Ophthalmic Vis Res. 2014 Jul-Sep;9(3):362-73. doi: 10.4103/2008-322X.143378. Review. PMID: 25667739
Role of angiogenesis in endodontics: contributions of stem cells and proangiogenic and antiangiogenic factors to dental pulp regeneration. Saghiri MA, Asatourian A, Sorenson CM, Sheibani N. J Endod. 2015 Jun;41(6):797-803. doi: 10.1016/j.joen.2014.12.019. Epub 2015 Jan 31. Review. PMID: 25649306
Expression of pigment epithelium-derived factor and thrombospondin-1 regulate proliferation and migration of retinal pigment epithelial cells. Farnoodian M, Kinter JB, Yadranji Aghdam S, Zaitoun I, Sorenson CM, Sheibani N. Physiol Rep. 2015 Jan 19;3(1). pii: e12266. doi: 10.14814/phy2.12266. Print 2015 Jan 1. PMID: 25602019
Effect of particle size on calcium release and elevation of pH of endodontic cements. Saghiri MA, Asatourian A, Orangi J, Lotfi M, Soukup JW, Garcia-Godoy F, Sheibani N. Dent Traumatol. 2015 Jun;31(3):196-201. doi: 10.1111/edt.12160. Epub 2015 Jan 8. PMID: 25571910
Expression of thrombospondin-1 modulates the angioinflammatory phenotype of choroidal endothelial cells. Fei P, Zaitoun I, Farnoodian M, Fisk DL, Wang S, Sorenson CM, Sheibani N. PLoS One. 2014 Dec 30;9(12):e116423. doi: 10.1371/journal.pone.0116423. eCollection 2014. PMID: 25548916
Angiogenesis in regenerative dentistry. Saghiri MA, Asatourian A, Sheibani N. Oral Surg Oral Med Oral Pathol Oral Radiol. 2015 Jan;119(1):122. doi: 10.1016/j.oooo.2014.09.023. Epub 2014 Sep 30. No abstract available. PMID: 25459357
Deletion of aldose reductase from mice inhibits diabetes-induced retinal capillary degeneration and superoxide generation. Tang J, Du Y, Petrash JM, Sheibani N, Kern TS. PLoS One. 2013 Apr 16;8(4):e62081. doi: 10.1371/journal.pone.0062081. Print 2013. PMID:23614016
Lack of Cyp1b1 promotes the proliferative and migratory phenotype of perivascular supporting cells. Palenski TL, Sorenson CM, Jefcoate CR, Sheibani N. Lab Invest. 2013 Jun;93(6):646-62. doi: 10.1038/labinvest.2013.55. Epub 2013 Apr 8. PMID: 23568032
Endoglin regulates the activation and quiescence of endothelium by participating in canonical and non-canonical TGF-β signaling pathways.Park S, Dimaio TA, Liu W, Wang S, Sorenson CM, Sheibani N. J Cell Sci. 2013 Mar 15;126(Pt 6):1392-405. doi: 10.1242/jcs.117275. Epub 2013 Feb 15. PMID: 23418351
Attenuation of choroidal neovascularization by β(2)-adrenoreceptor antagonism. Lavine JA, Sang Y, Wang S, Ip MS, Sheibani N. JAMA Ophthalmol. 2013 Mar;131(3):376-82. doi: 10.1001/jamaophthalmol.2013.1476. PMID:23303344
Pharmacological inhibition of mitochondrial carbonic anhydrases protects mouse cerebral pericytes from high glucose-induced oxidative stress and apoptosis. Shah GN, Price TO, Banks WA, Morofuji Y, Kovac A, Ercal N, Sorenson CM, Shin ES, Sheibani N. J Pharmacol Exp Ther. 2013 Mar;344(3):637-45. doi: 10.1124/jpet.112.201400. Epub 2012 Dec 17. PMID: 23249625
Aberrant production of extracellular matrix proteins and dysfunction in kidney endothelial cells with a short duration of diabetes. Grutzmacher C, Park S, Zhao Y, Morrison ME, Sheibani N, Sorenson CM. Am J Physiol Renal Physiol. 2013 Jan 1;304(1):F19-30. doi: 10.1152/ajprenal.00036.2012. Epub 2012 Oct 17. PMID: 23077100
Pericytes regulate vascular basement membrane remodeling and govern neutrophil extravasation during inflammation. Wang S, Cao C, Chen Z, Bankaitis V, Tzima E, Sheibani N, Burridge K. PLoS One. 2012;7(9):e45499. doi: 10.1371/journal.pone.0045499. Epub 2012 Sep 21. PMID: 23029055
Marrow-derived cells regulate the development of early diabetic retinopathy and tactile allodynia in mice. Li G, Veenstra AA, Talahalli RR, Wang X, Gubitosi-Klug RA, Sheibani N, Kern TS. Diabetes. 2012 Dec;61(12):3294-303. doi: 10.2337/db11-1249. Epub 2012 Aug 24. PMID: 22923475
Caspase-14: a novel caspase in the retina with a potential role in diabetic retinopathy. Al-Shabrawey M, Ahmad S, Megyerdi S, Othman A, Baban B, Palenski TL, Shin ES, Gurel Z, Hsu S, Sheibani N. Mol Vis. 2012;18:1895-906. Epub 2012 Jul 14. PMID: 2287611
Oligomeric forms of insulin amyloid aggregation disrupt outgrowth and complexity of neuron-like PC12 cells. Kachooei E, Moosavi-Movahedi AA, Khodagholi F, Ramshini H, Shaerzadeh F, Sheibani N. PLoS One. 2012;7(7):e41344. doi: 10.1371/journal.pone.0041344. Epub 2012 Jul 27. PMID: 22848469
Antibody-mediated retinal pericyte injury: implications for diabetic retinopathy. Li Y, Smith D, Li Q, Sheibani N, Huang S, Kern T, Nagaraj RH, Lin F. Invest Ophthalmol Vis Sci. 2012 Aug 13;53(9):5520-6. doi: 10.1167/iovs.12-10010. PMID: 22786897