Emery Bresnick, PhD

Portrait of Emery Bresnick, PhD
Professor
Cell and Regenerative Biology
Address: 
4009 WIMR
1111 Highland Ave
Madison, WI 53705
Telephone: 
(608) 265-6446
Focus Groups: 
Cancer Biology
Signal Transduction
Education: 
PhD, Pharmacology, The University of Michigan Medical School
Postdoctoral, National Institutes of Health, Laboratory of Molecular Biology
Research Summary: 
Stem Cell Biology, Epigenetics, Molecular Hematology, Vascular Biology, Cancer Biology: From Fundamental Mechanisms to Translational Medicine
Research Detail: 

We use multidisciplinary approaches to understand normal and malignant blood cell development/function (hematopoiesis), gene/chromosome regulation (including epigenetics), and vascular biology. Our team has expertise in genomics, proteomics, chemical genetics, and computational analysis, as well as powerful molecular, cellular, and biochemical science.

A major effort involves discovering mechanisms that regulate the genesis and function of hematopoietic stem and progenitor cells. Defining such mechanisms has enormous importance, as their disruption leads to the development of adult and pediatric blood cell cancers (leukemias, lymphomas, myelodysplastic syndrome) and additional blood cell disorders, including immunodeficiency. While hematopoietic stem cells are routinely transplanted to treat diverse diseases, their critical long-term repopulating activity is poorly understood and cannot be readily modulated for therapeutic application. Mechanistic insights can be exploited to develop novel approaches to therapeutically modulate hematopoietic stem cells, hematopoiesis, and blood cell malignancies.

Another program focuses on the transcriptional/epigenetic control of red blood cell development and function. Goals of this work include discovering the cause of red blood cell disorders, including anemias and hemoglobinopathies, developing translational strategies, and advancing fundamental knowledge in broadly important areas of biomedical science.

Since GATA-2 controls the emergence of hematopoietic stem cells from hemogenic endothelium, angiogenesis, vascular integrity, and developmental of the lymphatic system, we are studying how GATA-2 functions in the vascular system in normal and pathophysiological states.

Dr. Bresnick and his team are passionate about making biological and mechanistic discoveries, translating such discoveries into clinical insights and strategies, and training the next-generation of multidisciplinary researchers/scholars (undergraduates, graduate students, postdoctoral fellows).

Interested candidates can apply to:  ehbresni@wisc.edu

Selected Publications: 
Hewitt KJ, Johnson KD, Gao X, Keles S, Bresnick EH. The Hematopoietic Stem and Progenitor Cell Cistrome: GATA Factor-Dependent cis-Regulatory Mechanisms. Curr Top Dev Biol. 2016;118:45-76. doi: 10.1016/bs.ctdb.2016.01.002. Epub 2016 Feb 26. PubMed PMID: 27137654.
Gao X, Wu T, Johnson KD, Lahvic JL, Ranheim EA, Zon LI, Bresnick EH. GATA Factor-G-Protein-Coupled Receptor Circuit Suppresses Hematopoiesis. Stem Cell Reports. 2016 Mar 8;6(3):368-82. doi: 10.1016/j.stemcr.2016.01.008. Epub 2016 Feb 18. PubMed PMID: 26905203; PubMed Central PMCID: PMC4788764.
Tanimura N, Miller E, Igarashi K, Yang D, Burstyn JN, Dewey CN, Bresnick EH. Mechanism governing heme synthesis reveals a GATA factor/heme circuit that controls differentiation. EMBO Rep. 2016 Feb;17(2):249-65. doi: 10.15252/embr.201541465. Epub 2015 Dec 23. PubMed PMID: 26698166.
Johnson KD, Kong G, Gao X, Chang YI, Hewitt KJ, Sanalkumar R, Prathibha R, Ranheim EA, Dewey CN, Zhang J, Bresnick EH. Cis-regulatory mechanisms governing stem and progenitor cell transitions. Sci Adv. 2015 Sep 4;1(8):e1500503. doi: 10.1126/sciadv.1500503. eCollection 2015 Sep. PubMed PMID: 26601269; PubMed Central PMCID: PMC4643771.
Hewitt KJ, Kim DH, Devadas P, Prathibha R, Zuo C, Sanalkumar R, Johnson KD, Kang YA, Kim JS, Dewey CN, Keles S, Bresnick EH. Hematopoietic Signaling Mechanism Revealed from a Stem/Progenitor Cell Cistrome. Mol Cell. 2015 Jul 2;59(1):62-74. doi: 10.1016/j.molcel.2015.05.020. Epub 2015 Jun 11. PubMed PMID: 26073540; PubMed Central PMCID: PMC4499333.
DeVilbiss AW, Sanalkumar R, Hall BD, Katsumura KR, de Andrade IF, Bresnick EH. Epigenetic Determinants of Erythropoiesis: Role of the Histone Methyltransferase SetD8 in Promoting Erythroid Cell Maturation and Survival. Mol Cell Biol. 2015 Jun;35(12):2073-87. doi: 10.1128/MCB.01422-14. Epub 2015 Apr 8. PubMed PMID: 25855754; PubMed Central PMCID: PMC4438249.
McIver SC, Kang YA, DeVilbiss AW, O'Driscoll CA, Ouellette JN, Pope NJ, Camprecios G, Chang CJ, Yang D, Bouhassira EE, Ghaffari S, Bresnick EH. The exosome complex establishes a barricade to erythroid maturation. Blood. 2014 Oct 2;124(14):2285-97. doi: 10.1182/blood-2014-04-571083. Epub 2014 Aug 12. PubMed PMID: 25115889; PubMed Central PMCID: PMC4183988.
Katsumura KR, Yang C, Boyer ME, Li L, Bresnick EH. Molecular basis of crosstalk between oncogenic Ras and the master regulator of hematopoiesis GATA-2. EMBO Rep. 2014 Sep;15(9):938-47. doi: 10.15252/embr.201438808. Epub 2014 Jul 23. PubMed PMID: 25056917; PubMed Central PMCID: PMC4198037.
Yamazaki H, Suzuki M, Otsuki A, Shimizu R, Bresnick EH, Engel JD, Yamamoto M. A remote GATA2 hematopoietic enhancer drives leukemogenesis in inv(3)(q21;q26) by activating EVI1 expression. Cancer Cell. 2014 Apr 14;25(4):415-27. doi: 10.1016/j.ccr.2014.02.008. Epub 2014 Apr 3. PubMed PMID: 24703906; PubMed Central PMCID: PMC4012341.
Gao X, Johnson KD, Chang YI, Boyer ME, Dewey CN, Zhang J, Bresnick EH. Gata2 cis-element is required for hematopoietic stem cell generation in the mammalian embryo. J Exp Med. 2013 Dec 16;210(13):2833-42. doi: 10.1084/jem.20130733. Epub 2013 Dec 2. PubMed PMID: 24297994; PubMed Central PMCID: PMC3865483.
Hsu A, Johnson KD, Falcone EL, Sanalkumar R, Sanchez L, Hickstein DD, Cuellar-Rodriguez J, Lemieux JE, Zerbe CS, Bresnick EH, and Holland SM. GATA2 haploinsufficiency caused by mutations in a conserved intronic element leads to MonoMAC syndrome. Blood March 15 [Epub ahead of print], 2013.
Johnson K.D., Hsu A.P., Ryu M.-J., Wang J., Gao X., Boyer M.E., Keles S., Lee Y., Calvo K.R., Zhang J., Holland S.M., and Bresnick E.H. (2012) Cis-element mutated in GATA2-dependent immunodeficiency governs hematopoiesis and vascular integrity. J. Clin. Invest., In Press.
Kang YA, Sanalkumar R, O'Geen H, Linnemann AK, Chang CJ, Bouhassira EE, Farnham PJ, Keles S, Bresnick EH. Autophagy driven by a master regulator of hematopoiesis.(Abstract)Mol Cell Biol. 2012 Jan;32(1):226-39. Epub 2011 Oct 24.
Bresnick EH, Katsumura KR, Lee HY, Johnson KD, Perkins AS. Master regulatory GATA transcription factors: mechanistic principles and emerging links to hematologic malignancies.(Abstract) Nucleic Acids Res. 2012 Apr 5. [Epub ahead of print]
Chung D, Kuan PF, Li B, Sanalkumar R, Liang K, Bresnick EH, Dewey C, Keles S. Discovering transcription factor binding sites in highly repetitive regions of genomes with multi-read analysis of ChIP-seq data. PLOS Comput Biol 7: e1002111, 2011. PMCID PMC3136429
Linnemann AK, O'Geen H, Keles S, Farnham PJ, Bresnick EH. Genetic framework for GATA factor function in vascular biology.(Abstract) Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):13641-6. Epub 2011 Aug 1.
Bresnick EH, Lee HY, Fujiwara T, Johnson KD, and Keles S. (2010) GATA switches as developmental drivers. (minireview) J. Biol. Chem. 285:31087-31093
Li L, Jothi R, Cui K, Lee JY, Cohen T, Gorivodsky M, Tzchori I, Zhao Y, Hayes SM, Bresnick EH, Zhao K, Westphal H, Love PE. (2011) Nuclear adaptor Ldb1 regulates a transcriptional program essential for the maintenance of hematopoietic stem cells. Nat Immunol. 2011 Feb;12(2):129-36. Epub 2010 Dec 26
Snow JW, Trowbridge JJ, Fujiwara T, Emambokus NE, Grass JA, Orkin SH, Bresnick EH. A single cis element maintains repression of the key developmental regulator Gata2.(Abstract) PLoS Genet. 2010 Sep 9;6(9). pii: e1001103.
Fujiwara T, O'Geen H, Keles S, Blahnik K, Linnemann AK, Kang YA, Choi K, Farnham PJ, Bresnick EH. Discovering hematopoietic mechanisms through genome-wide analysis of GATA factor chromatin occupancy.(Abstract) Mol Cell. 2009 Nov 25;36(4):667-81.