Adel M. Talaat, MVSc, PhD

Portrait of Adel M. Talaat, MVSc, PhD
Pathobiological Sciences
1656 Linden Dr
Madison, WI 53706
(608) 262-2861
Focus Groups: 
PhD, Univeristy of Maryland School of Medicine
MVSc, Faculty of Veterinary Medicine, Cairo Univeristy, Egypt
Research Summary: 
Genomic and functional analyses of tuberculosis and paratuberculosis to understand pathogenesis and develop novel vaccines
Research Detail: 

The research in my laboratory involves the use of innovative approaches to understand the basics of bacterial pathogenesis and evolution on a genome-wide scale. Definitely, we advocate the idea of “useful genomics” where genome-wide protocols are used to better understand the disease process or to discover novel vaccine and drug targets.

Currently, we are exploiting the genome of Mycobacterium tuberculosis, the causative agent of human tuberculosis, to better understand the critical stages of persistent tuberculosis. In particular, we employ the array differential gene expression (ADGE) profiling to unravel the transcriptional machinery that underlies mycobacterial virulence and the nature of host-pathogen interactions. We also use gene-targeted mutational analysis alongside cellular, biochemical and molecular techniques to determine the role of a novel genomic island expressed only during infection (iVEGI) in mycobacterial survival.

Other members of the laboratory investigate the pathogenesis and evolution of M. avium ss. paratuberculosis (M. ap), the causative agent of Johne’s disease in cattle. In particular, we address questions related to the very early stages (few hours) of intestinal invasion by M. ap bacilli as well as virulence factors associated with chronic infection. With the availability of the genome sequences of members of M. avium complex, we are currently using comparative genomic hybridization, optical mapping and statistical modeling to reconstruct the evolutionary history of M. avium complex. We are also evaluating the impact of the genome synteny on gene expression and bacterial pathogenesis.

Selected Publications: 
Bannantine JP, Wu CW, Hsu C, Zhou S, Schwartz DC, Bayles DO, Paustian ML, Alt DP, Sreevatsan S, Kapur V, Talaat AM. Genome sequencing of ovine isolates of Mycobacterium avium subspecies paratuberculosis offers insights into host association. BMC Genomics. 2012 Mar 12;13:89.
Bartpho T, Wongsurawat T, Wongratanacheewin S, Talaat AM, Karoonuthaisiri N, Sermswan RW. Genomic islands as a marker to differentiate between clinical and environmental Burkholderia pseudomallei. PLoS One. 2012;7(6):e37762. Epub 2012 Jun 1.
Ghosh P, Hsu C, Alyamani EJ, Shehata MM, Al-Dubaib MA, Al-Naeem A, Hashad M, Mahmoud OM, Alharbi KB, Al-Busadah K, Al-Swailem AM, Talaat AM. Genome-wide analysis of the emerging infection with Mycobacterium avium subspecies paratuberculosis in the Arabian camels (Camelus dromedarius). PLoS One. 2012;7(2):e31947. Epub 2012 Feb 29.
Abomoelak B, Marcus SA, Ward SK, Karakousis PC, Steinberg H, Talaat AM. Characterization of a novel heat shock protein (Hsp22.5) involved in the pathogenesis of Mycobacterium tuberculosis. J Bacteriol. 2011 Jul;193(14):3497-505. Epub 2011 May 20.
Hsu CY, Wu CW, Talaat AM. Genome-Wide Sequence Variation among Mycobacterium avium Subspecies paratuberculosis Isolates: A Better Understanding of Johne's Disease Transmission Dynamics. Front Microbiol. 2011;2:236. Epub 2011 Dec 9.
Bannantine JP, Talaat AM. Genomic and transcriptomic studies in Mycobacterium avium subspecies paratuberculosis. Vet Immunol Immunopathol. 2010 Dec 15;138(4):303-11. Epub 2010 Nov 2. Review.
Wynne JW, Seemann T, Bulach DM, Coutts SA, Talaat AM, Michalski WP. Resequencing the Mycobacterium avium subsp. paratuberculosis K10 genome: improved annotation and revised genome sequence. J Bacteriol. 2010 Dec;192(23):6319-20. Epub 2010 Sep 24.
Ward SK, Abomoelak B, Hoye EA, Steinberg H, Talaat AM. CtpV: a putative copper exporter required for full virulence of Mycobacterium tuberculosis. Mol Microbiol. 2010 Sep;77(5):1096-110. doi: 10.1111/j.1365-2958.2010.07273.x.
Ward SK, Abomoelak B, Marcus SA, Talaat AM. Transcriptional profiling of mycobacterium tuberculosis during infection: lessons learned. Front Microbiol. 2010;1:121. Epub 2010 Nov 18.
Wu, C-W, Schmoller, S.K., Bannantine, J.P., Ecksteinc, T.M., Inaminec, J.M., Livesey, M., Albrecht, R., Talaat, A.M. A novel cell wall lipopeptide is important for biofilm formation and pathogenicity of Mycobacterium avium subspecies paratuberculosis. Microbial. Pathogen. 2009. Online.